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IC50 LABWEB TRIAL · DOSE–RESPONSE ANALYSIS
LOCAL BROWSER ANALYSIS · NOT SAVED

ASSAY WORKBENCH / 4-PARAMETER LOGISTIC FIT

Turn dose–response data
into clear IC50 results.

Analyze antiviral, replicon, cell viability, cytotoxicity, and other inhibition assays with replicate-aware plots, fit diagnostics, and unit-safe reporting.

TRIAL WORKFLOW01

Paste or upload one assay

02

Review the fit and diagnostics

03

Unlock exports and comparisons on Mac

WEB TRIALone assay · session only
02
FIT & REVIEW4PL · UNWEIGHTED LEAST SQUARES
EXPORTS IN MAC APP ↗
RELATIVE IC5012.789 nM4PL MIDPOINT · APPROX. 95% CI 11.638–14.054 nMWITHIN TESTED RANGE
HILL SLOPE1.184
BOTTOM / TOP2.6 / 100.4%CHECK PLATEAU
R²0.999
PLOT SETTINGS
TRIAL VIEW · 16:9Publication styling and all file exports are included in the Mac app.
CHART ELEMENTS
RDV Replicon AssayDose-response plot with relative IC50 results. Included and excluded observations shown in the current point view can be selected with Enter or Space for review.RDV Replicon AssayIC50 LAB WEB TRIAL · EXPORTS DISABLED02550751001520100CONCENTRATION (nM)INHIBITION (%)RELATIVE IC₅₀R² 0.99912.789 nM
PASS

Fit reliability
Converged in 161 iterations · 4/3 doses below/above IC₅₀

PASS

Approx. 95% IC₅₀ interval
11.638–14.054 nM · Wald, log₁₀ scale

PASS

Replicate coverage
6/7 non-zero doses have repeat observations

PASS

Replicate agreement
Max spread 3.7 points at 30.000 nM

PASS

Observed plateau support
Lowest- and highest-dose means approach fitted plateaus

INFO

Model selection
R² 0.999 · SSE 20.1 · AICc 17.5

FIT NOTES

RELATIVE IC₅₀ · The reported value is the 4PL midpoint between the fitted bottom and top; it is not necessarily an absolute 50% response.

UNCONSTRAINED PLATEAU · The fitted top is 100.4%. This may reflect normalized assay variability; apply a constraint only when scientifically justified.